LOCUS       sequence-69256-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codes for the SSA-TLR with the target site for I-Ani I in a 
            lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pCVL.SSA TLR (Ani)
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     236..257
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(300..353)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
       61 tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggttcacgc tagcctttac
      121 agagaaacct cctcaggcgg ccgcttgccg gtggtgcaga tgaacttcag ggtcagcttg
      181 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
      241 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacg
      301 gggcccggat tctcctccac gtcaccgcat gttagaagac ttcctctgcc ctctctagac
      361 cacagtcgct cgccgcgcaa aatcgagatc gacatcgtgc cgtgcatgcc tatgttgcgc
      421 atgaattcca gatggacggg cgagacgctg cgcaggatgg cgaagctaag atcaatcggc
      481 cgcccggtga ccggattgag gtctggggtg accggcaccg gccgataatt gatatcggga
      541 atgatccgta ccgggttgat ggtatagagc cgacgggcct gcgccggcac ggttgaggca
      601 ggatagtgca ggcctagttt tgactcgacc tcggcgcacc gatcctctgc gatcacttcg
      661 ccgctgaagt cggaggcgaa gcgatagatc atcacccgat cgaagccggt aatcttccgc
      721 acctcttgcg ccgcggcggc gcaggcgctt tccaaggttt cggcggcctg caggcggcgg
      781 atggcgctgt tggtgcggcg gaagaacgcc tgcggctcgg cgacgtcccg ctggggaggc
      841 tcgagctcga ggaagatgag ctgatcatgg cgatgccagg agccgatgaa gcctgcgtcc
      901 tttcgcatcg tgaagccgac agtgatcggt gctccgacgg ccgccccggg
//
