LOCUS       sequence-69258-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codes for the SSA-TLR with the target site for the CCR5 TALEN with 
            the spacer region corresponding to I-Sce I in a lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pCVL.SSA TLR (CCR5 TALEN Sce Spacer)
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     269..290
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(333..386)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
       61 tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggttcacgc tagccttcca
      121 gaattgatac ttagggataa cagggtaata agctgcaggt gtaatgaaag cggccgcttg
      181 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      241 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      301 accccggtga acagctcctc gcccttgctc acggggcccg gattctcctc cacgtcaccg
      361 catgttagaa gacttcctct gccctctcta gaccacagtc gctcgccgcg caaaatcgag
      421 atcgacatcg tgccgtgcat gcctatgttg cgcatgaatt ccagatggac gggcgagacg
      481 ctgcgcagga tggcgaagct aagatcaatc ggccgcccgg tgaccggatt gaggtctggg
      541 gtgaccggca ccggccgata attgatatcg ggaatgatcc gtaccgggtt gatggtatag
      601 agccgacggg cctgcgccgg cacggttgag gcaggatagt gcaggcctag ttttgactcg
      661 acctcggcgc accgatcctc tgcgatcact tcgccgctga agtcggaggc gaagcgatag
      721 atcatcaccc gatcgaagcc ggtaatcttc cgcacctctt gcgccgcggc ggcgcaggcg
      781 ctttccaagg tttcggcggc ctgcaggcgg cggatggcgc tgttggtgcg gcggaagaac
      841 gcctgcggct cggcgacgtc ccgctgggga ggctcgagct cgaggaagat gagctga
//
