LOCUS       sequence-69266-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses K227M nickase variant of I-Ani I nuclease in mammalian 
            cells with BFP tracker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             64..90
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             94..114
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccgctcgagc cgccaccatg
       61 ggatatccat acgatgtccc agattatgcg ccacctaaga agaaacgcaa agtcgaattc
      121 ggcagcgatc tgacctacgc ctatctggtg ggcctgtacg agggcgacgg atatttttcc
      181 atcactaaaa agggcaagta cctgacctat gagctgggaa ttgaactgtc tatcaaggat
      241 gtgcagctga tctacaagat caagaagatc ctggggatcg gcattgtgag cttcaggaag
      301 agaaacgaga ttgaaatggt ggccctgagg atcagggata agaatcacct gaaatctaag
      361 attctgccca tcttcgagaa gtatcctatg tttagtaaca aacagtacga ctatctgagg
      421 tttagaaatg ctctgctgag cggcatcatc tacctggagg atctgccaga ctatacccgg
      481 tccgacgagc ccctgaacag catcgaatcc atcattaata catcttactt cagtgcctgg
      541 ctggtgggct tcatcgaggc tgaagggtgc ttctctgtgt acaaactgaa caaggacgat
      601 gactatctga ttgccagttt tgatatcgct cagagggatg gagacatcct gattagcgcc
      661 atcagaaagt acctgtcctt caccacaaag gtgtatctgg acaaaacaaa ttgtagcaaa
      721 ctgaaggtca ctagcgtgcg ctccgtggag aacatcatta agttcctgca gaatgctcct
      781 gtgaaactgc tgggcaacat gaagctgcag tacaaactgt ggctgaagca gctgcggaaa
      841 atctctcgct acagtgaaaa aatcaagatt ccatccaatt attaacctgc aggtcgagca
      901 tgcatctag
//
