LOCUS       sequence-69268-e        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses K227M nickase variant of I-Ani I nuclease in mammalian 
            cells with BFP tracker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             182..883
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
       61 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
      121 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccttac cggtcgccac
      181 catgagcgag ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga
      241 caaccatcac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
      301 catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
      361 tagcttcctc tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt
      421 caagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg
      481 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
      541 gatcagaggg gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg
      601 ggaggccttc accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat
      661 ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac atcaagacca catatagatc
      721 caagaaaccc gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga
      781 aagaatcaag gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag
      841 atactgcgac ctccctagca aactggggca caagcttaat tgattctaga gtcgaccgag
      901 catcttaccg ccatttatac ccatatttgt tctgtttttc ttgatttggg tatacattta
      961 aatgttaata gaacaaaatg gtggggcaat catttacatt tttagggata tgtaattact
     1021 agttcaggtg tattgccaca agacaaacat gttaagaaac tttc
//
