LOCUS       sequence-69293-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PcHA-Bmal backbone used for the above mutation. Ref.Genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Huang N, Chelliah Y, Shan Y, Taylor CA, Yoo SH, Partch C, Green CB, 
            Zhang H, Takahashi JS
  TITLE     Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional 
            activator complex.
  JOURNAL   Science. 2012 Jul 13;337(6091):189-94. doi: 10.1126/science.1222804.
            Epub 2012 May 31.
  PUBMED    22653727
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1222804"; journalName: "Science"; date: 
            "2012-07-13- 13"; volume: "337"; issue: "6091"; pages: "189-94"
COMMENT     Sequence Label: pCDNA3-HA/Bmal V435R
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             81..107
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttcgat cgtcgacatg tacccatacg atgttccaga ttacgcttct agaggatccg
      121 cggaccagag aatggacatt tcctcaacca tcagcgactt catgtctccg ggccccaccg
      181 acctactctc cggttccctg ggcaccagtg gtgtggactg caatcgcaag aggaaaggca
      241 gtgccactga ctaccaagaa agtatggaca cagacaaaga tgaccctcat ggaaggttag
      301 aatatgcaga acaccaagga aggatcaaga atgcaaggga ggcccacagt cagattgaaa
      361 agaggcgtcg ggacaaaatg aacagtttca ttgatgaatt ggcttctttg gtaccaacat
      421 gcaatgcaat gtccaggaag ttagataaac tcaccgtgct aaggatggct gttcagcaca
      481 tgaaaacttt gagaggtgcc accaacccat acacagaagc aaactacaag ccaacatttc
      541 tatcagatga cgaactgaaa cacctaattc tcagggcagc agatggattt ttgtttgtcg
      601 taggatgtga ccgagggaag atcctctttg tctccgagtc tgtcttcaag atcctcaatt
      661 atagccagaa tgaccttatt ggccagagct tgtttgacta cctgcatcca aaagatattg
      721 ccaaagttaa ggaacagcta tcttcctcgg acactgcgcc ccgggagcga ctcattgatg
      781 ccaagactgg acttccggtt aaaacggata taacccctgg gccctcccgg ctatgctctg
      841 gagcccgccg ctctttcttc tgtagaatga agtgcaacag gccttcagta aaggtggaag
      901 ataaggactt cg
//
