LOCUS       sequence-69297-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mClock fragment cloned into pfastbacHTb vector with Hexa His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Huang N, Chelliah Y, Shan Y, Taylor CA, Yoo SH, Partch C, Green CB, 
            Zhang H, Takahashi JS
  TITLE     Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional 
            activator complex.
  JOURNAL   Science. 2012 Jul 13;337(6091):189-94. doi: 10.1126/science.1222804.
            Epub 2012 May 31.
  PUBMED    22653727
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1222804"; journalName: "Science"; date: 
            "2012-07-13- 13"; volume: "337"; issue: "6091"; pages: "189-94"
COMMENT     Sequence Label: pfastbacHTb/mClock26-384
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..102
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             124..144
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg gcgcggatct
       61 cggtccgaaa ccatgtcgta ctaccatcac catcaccatc acgattacga tatcccaacg
      121 accgaaaacc tgtattttca gggcgccatg gtggaagaag atgacaagga caaagcaaaa
      181 agagtatcta gaaacaaatc agaaaagaaa cgtagagatc agttcaatgt cctcattaag
      241 gagctggggt ctatgcttcc tggtaacgcg agaaagatgg acaagtctac tgttctacag
      301 aagagcattg attttttgcg caaacataaa gagaccactg cacagtcaga tgctagtgag
      361 attcgacagg actggaaacc cacattcctt agtaatgaag agtttacaca gttaatgtta
      421 gaggctcttg atggtttttt tttagcgatc atgacagatg gaagtataat atatgtatct
      481 gagagtgtaa cttcgttact tgaacattta ccatctgatc ttgtggatca aagtatattt
      541 aattttatcc cagagggaga acattcagag gtttataaga tactctctac tcatctgctg
      601 gaaagtgact cattaacccc tgagtactta aaatcaaaaa atcagttaga attctgttgt
      661 cacatgcttc gaggaacaat agacccaaag gagccatcca cctatgaata tgtgagattt
      721 ataggaaatt ttaaatcttt aaccagtgta tcaacttcaa cacacaatgg ttttgaagga
      781 actatacaac gcacacatag gccttcttat gaagatagag tttgttttgt agctactgtc
      841 agattagcta cacctcagtt catcaaggaa atgtgtactg ttgaagaacc aaatgaagag
      901 tttacatcta gacacagttt agaatggaag
//
