LOCUS       sequence-69305-e        8611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses W37A mutated Cic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Kim E, Lu HC, Zoghbi HY, Song JJ
  TITLE     Structural basis of protein complex formation and reconfiguration by
            polyglutamine disease protein Ataxin-1 and Capicua.
  JOURNAL   Genes Dev. 2013 Mar 15;27(6):590-5. doi: 10.1101/gad.212068.112.
  PUBMED    23512657
REFERENCE   2  (bases 1 to 8611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.212068"; journalName: "Genes Dev"; date: "2013-03-15- 
            15"; volume: "27"; issue: "6"; pages: "590-5"
COMMENT     Sequence Label: Myc-CICf_W37A
FEATURES             Location/Qualifiers
     source          1..8611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     485..505
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     531..555
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          672..768
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      2896..2905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5750..5884
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5774..5793)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5828..5847
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5995..6011)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5995..6011)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6008..6030)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6019..6035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6043..6049,6050..6067,6068..6073))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6088..6109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6226..6243)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6397..6985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6477..6496)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7156..7947,7948..8016))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7779..7798
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8017..8121)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8189..8207
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8245..8267)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8367..8386
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8580..8602
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8594..8611
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8595..8611
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggcatcaatg
      841 cagaagctga tctcagagga ggacctgctt atggccatgg aggcccgaat tcggtcgacc
      901 atgtactcgg cccacaggcc cctgataccc gcgtctggcg cggcctctcg tggcctcggc
      961 atgttcgtgt ggacaaatgt cgaacctcgt tctgtggctg tgttccccgc gcactcctta
     1021 gtccccttcc tggcacccag ccagcccgac ccctctgtgc agcctagtga ggcccagcaa
     1081 cctgccagcc accctgtggc ctccaaccag agcaaagaac ctgctgagtc tgctgctgtt
     1141 gctcatgagc agccaccagg agggacgggg ggtgctgacc ctggacggcc ccctggagca
     1201 gtgtgccctg agagcccagg gcctggacct ccacttactt tgggtggtgt ggatcctggt
     1261 aaaagtctcc cccccaccac tgaggaggag gctcctggcc ctccaggaga gccccggctg
     1321 gacagtgaga ccgagagtga tcatgatgat gccttcctct ccatcatgtc tcctgagatt
     1381 cagttgcctc tgccacctgg aaagcgccgc acccagtctc tgagtgcctt gcccaaggaa
     1441 cgagactcat cttctgaaaa ggatggacga agtcctaaca agcgggagaa ggaccatatt
     1501 cgtcggccca tgaatgcctt catgatcttc agcaagcggc accgggcctt ggtccaccag
     1561 cggcacccca accaggacaa ccggactgtc agcaagatcc tgggcgagtg gtggtacgcc
     1621 ctggggccca aagagaagca gaaataccac gacctggcct tccaggtgaa agaggcccac
     1681 ttcaaggccc acccagattg gaaatggtgc aacaaggacc gaaagaagtc cagctcagag
     1741 gccaagcctg cgagcctggg tctggcagga gggcacaagg agacgcggga acggagcatg
     1801 tcggagacgg gaactgctgc tgcccctggg gtgtcttctg agctcctgtc tgttgctgcc
     1861 cagacacttt tgagctcgga caccaaggtt ccagggagtg gcccctgtgg agcagaacgg
     1921 ctacacgcag ttggggcacc tggctcagcc cgacccagag ccttctccca cagtggggtg
     1981 cacagtcttg atggtgggga agtagacagc caagcacttc aagaactgac ccagatggtt
     2041 tctggccccg catcatactc cggcccaaag ccttcccccc agtatggtgc tccaggatct
     2101 tttgcagctc ctggtgaagg aggtaccttg gccactagtg ggcggcctcc actgttgccc
     2161 tcccgagcct ctcgttccca gcgtgcagcc agtgaggaca tgaccagtga tgaggagcgg
     2221 atggtcatct gtgaggaaga aggggatgat gatgtcattg ctgatgacag ctttggcacc
     2281 accgacattg atctcaagtg caaggaacga gtgactgaca gtgaaagtgg agacagctct
     2341 ggggaggacc cagagggtaa caagggcttt ggccgtaagg tgttctcacc tgtcatccgc
     2401 tcctccttta cccattgccg tccaaccctg gaccctgagc ctccagggcc cccggatcca
     2461 cctgcagcct tcagcaaagg ctacggtccc accccatcat cctcctcttc acctgcttcc
     2521 acctcagtct cagtctccac ctccttttca ctgggctctg gaacctttaa gacccaggag
     2581 tctggtcagg gcagcacagc ggtgccacta cggcccccac cccctggagc cgggggccca
     2641 gcaacacctt ccaaggccac tcggtttcct cctacggatt ctgccacctt tcggcgcaag
     2701 agacctgaaa gtgttggtag ccttgaagca ccaggcccct ctgtcattgc agcacctccc
     2761 agtgggggag gaaaccttct gcagacactg gttctgcctc cgagcaagga ggatcgggag
     2821 ggtacacgag tgccctcagc cccagcccca ccactggctt atggggctcc cgcagcccct
     2881 ctgtgccgcc ctgctgccac catggtcacc aatgtggtac ggcctgtcag cagcactcct
     2941 gtgcccattg cctctaagcc ctttcccacc tctggtcggg ctgaggcatc ttcaaatgac
     3001 atagcaggtg ccaggactga aatgggcaca ggatcccggg tgcctggggg ctccccaatg
     3061 ggtgtcagtt tagtgtattc agataagaag tcagcagcag cagccacctc gccagctcca
     3121 catttggtag ctggaccttt attgggcact gtggggaagg cacctgctac tgtcaccaac
     3181 ttgctggtag gcaccccagg ctatggggct cctgcatcgc ctgctgttca gtttattgcc
     3241 cagggagccc caggcagtgc aacccctgca ggctcaggag caagtactgg gagtggcccc
     3301 aatgggccag tacccctggg catcctgcag ccaggtgccc taggcaaggc tgggggaatc
     3361 acacaggtgc agtacatcct gcccacactg ccccagcagc ttcaagtggc acctgcccca
     3421 gcaccagccc ctgggaccaa ggcagcagct cccagtggcc ctgcacccac caccagcatc
     3481 cgtttcaccc tccctccggg cacctcgacc aacggcaagg tcctggctgc cactgcaccc
     3541 actgctggca tccctatcct gcagtccgta ccctcagccc caccccctaa agcccagtca
     3601 gtttctcctg tccaggccac accttcaggt ggctcagccc agctgctgcc tgggaaggtg
     3661 ctagtccccc tggctgcccc tagcatgtca gttcgaggtg gaggggctgg tcagccactg
     3721 cccctggtta gctcgccttt ctcagtacct gtccaaaatg gtgcccaaca acctagcaag
     3781 attatccagc tgactcctgt gcctgtgagc acacctagtg gcctggtacc acccctgagc
     3841 ccagctacaa tgccgggacc cacatcacag cctcagaagg tcctgttgcc ctcttccaca
     3901 agaatcacct atgtacagtc agcaggtggg cacactctgc ctctaggcac cagttctgca
     3961 tgcagtcaga ctggaacagt gacctcatat gggcccacca gctctgtagc tctgggcttc
     4021 acatctttgg ggcccagtgg tcctgccttt gtacagcctc tgctctcagc aggccaagct
     4081 cctttgctgg ctcctggcca ggtgggcgtg tcacctgtgc ctagtcccca gttgcctcct
     4141 gcctgtacag cctctggagg tcctgtcata acagcatttt accctggcag ccctgcaccc
     4201 acttcagcac ccctgggccc accttcccaa gctccgccaa gcctggtcta cactgtagcc
     4261 accagtacca ctccacctgc tgctaccatt ctgcccaagg gcccaccagc ctctgccact
     4321 gccactccag cccctactag tcctttccct agcgccacag caggctccat gacctacagc
     4381 ttagtggctc ccaaagctca gcgacccagc ccaaaagctc cccagaaagt aaaggcagcc
     4441 attgccagca ttcctgtggg gtcttttgaa tcgggtacta ctgggcggcc tggatctaca
     4501 ccccgacagt cttcagactc tggcgtagcc agagagcctg ctgccccaga atcagaactt
     4561 gaggggcagc ccacaccccc agctccccca ccccccacag agacctggcc tcccactgcc
     4621 cggagcagtc ccccaccccc cttgcctgct gaggagcgac ctggcactaa aggccctgag
     4681 actgccagca aattccccag ctcatcttca gactggcgag ttcctgggct gggcctggag
     4741 agtcgtgggg agcctcctac ccctcccagc ccagctccag ccacaggccc cagtggaagc
     4801 agcagtggca gcagcgaggg cagtagtggg agggcagctg gggacacacc cgagcgcaag
     4861 gaagtcacta gttctggcaa gaagatgaag gtgcggcccc cacccctgaa gaagaccttt
     4921 gactctgtgg acaacagggt cctgtcagaa gtggactttg aagagcggtt tgctgagctg
     4981 ccggagttta gaccagagga ggtgctgccc tcacccaccc tgcagtctct ggccacctcg
     5041 cctcgggcta tcctcggctc ctaccgaaag aagaggaaga attccacgga cctagactca
     5101 gcacctgaag atcccacctc acccaagcgc aagatgagga gacgttcgag ctgcagctca
     5161 gagcccaaca cccccaagag tgccaagtgc gagggggaca tcttcacctt tgaccgcaca
     5221 ggtactgaga cagaggatgt gctcggggag ctggagtatg agaaggtgcc ctactcatca
     5281 ctgcggcgca ccctggacca acggcgggcc ctggtcatgc agctcttcca ggaccatggc
     5341 ttcttcccat cagcccaggc cacagcagcc ttccaggccc gctacgcaga catcttccca
     5401 tccaaggtgt gtctgcaatt aaagatccga gaggtccgcc agaagatcat gcaggcagcc
     5461 actcccacag agcagccccc tggggctgaa gcccccctcc ctggaccacc ccctactggc
     5521 atggctgcta ctcctgtccc cactcccagc cctgctgggg gccctgaccc cacctctcca
     5581 ggctcggact ctggcactgc ccaagttgcc ccgccactgc ctccaccccc agagcctggg
     5641 cctggacagc ctggctggga gggggctccc cagccctcac cacctccctc tggcccttcc
     5701 acagctgcca caggcaggtg aagatctctc gaggtaccgc ggccgcgggg atccagacat
     5761 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     5821 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     5881 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     5941 tttttcggat cctctagagt cgatctgcag gcatgctagc ttggcgtaat catggtcata
     6001 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6061 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6121 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6181 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6241 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6301 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6361 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6421 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6481 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6541 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6601 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6661 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6721 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6781 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     6841 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6901 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6961 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7021 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7081 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7141 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7201 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7261 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7321 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7381 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7441 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7501 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7561 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7621 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7681 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7741 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7801 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7861 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7921 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7981 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8041 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8101 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     8161 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     8221 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     8281 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     8341 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     8401 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     8461 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     8521 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     8581 ccagtcacga cgttgtaaaa cgacggccag t
//
