LOCUS       sequence-69343-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag, Myc tagged Nbr in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Han BW, Hung JH, Weng Z, Zamore PD, Ameres SL
  TITLE     The 3'-to-5' exoribonuclease Nibbler shapes the 3' ends of microRNAs
            bound to Drosophila Argonaute1.
  JOURNAL   Curr Biol. 2011 Nov 22;21(22):1878-87. Epub 2011 Nov 3.
  PUBMED    22055293
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2011-11-22"; volume: "21(22)"; pages: "1878-87. Epub 
            2011 Nov 3"
COMMENT     Sequence Label: pAFM-NbrWT
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             291..669
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(325..344)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgtctggatc ccgtttaaac tacgcgtaat tcaaacaggg ttctggcgtc gttctcgtac
       61 tgttttcccc aggccagtgc tttagcgtta ttgaaaaagg aagagtatga gtattcaaca
      121 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      181 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      241 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      301 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      361 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      421 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      481 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
      541 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
      601 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
      661 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
      721 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
      781 tggctggttt attgctgata aatctggagc cg
//
