LOCUS       sequence-69344-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector containing SFFV promoter, dCas9 that is 
            fused to 2x NLS and tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pHR-SFFV-dCas9-BFP
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(95..793)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             complement(833..853)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(860..880)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atctttcaca aattttgtaa tccagaggtt gattatcgat aagcttgata tcaagcttgc
       61 atgcctgcag gtcgactcta gagtcgcggc cttactaatt aagcttgtgc cccagtttgc
      121 tagggaggtc gcagtatctg gccactgcca cctcgtgctg ctcgacgtag gtctcgttgt
      181 tggcctcctt gattctttcc agtctgtagt ccacatagta gacgccaggc atcttgaggt
      241 tcttagcggg tttcttggat ctatatgtgg tcttgatgtt tgcgatcaga tggctcccgc
      301 ccacgagctt cagggccatg tcgtttctgc cttccaggcc gccgtcagcg gggtacagcg
      361 tctcggtgaa ggcctcccag ccgagtgttt tcttctgcat cacagggccg ttggatgtga
      421 agttcacccc tctgatcttg acgttgtaga tgaggcagcc gtcctggagg ctggtgtcct
      481 gggtagcggt cagcacgccc ccgtcttcgt atgtggtgac tctctcccat gtgaagccct
      541 cagggaagga ctgcttgaag aagtcgggga tgccctgggt gtggttgatg aaggtcttgc
      601 tgccgtagag gaagctagta gccaggatgt cgaaggcgaa ggggagaggg ccgccctcga
      661 ccaccttgat tctcatggtc tgggtgccct cgtagggctt gccttcgccc tcggatgtgc
      721 acttgaagtg atggttgtcc acggtgccct ccatgtacag cttcatgtgc atgttctcct
      781 taatcagctc gctggatccg ctgctgccgt tgctcccact accaatgccg tccactttcc
      841 gctttttctt aggatcttcc accttgcgtt ttttcttggg ggagccgctg ccc
//
