LOCUS       sequence-69346-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector containing SFFV promoter, dCas9 that is 
            fused to 2x NLS and tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pHR-SFFV-dCas9-BFP
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..56)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(63..83)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(96..122)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(102..128)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccgctgctgc cgttgctccc actaccaatg ccgtccactt tccgcttttt cttaggatct
       61 tccaccttgc gttttttctt gggggagccg ctgcccaggc tggcataatc gggcacgtca
      121 tagggatagg cgtcgcctcc cagctgagac aggtcgatcc gtgtctcgta caggccggtg
      181 atgctctggt ggatcagggt ggcgtccagc acctctttgg tgctggtgta cctcttccgg
      241 tcgatggtgg tgtcaaagta cttgaaggcg gcaggggctc ccagattggt cagggtaaac
      301 aggtggatga tattctcggc ctgctctctg ataggcttgt ctctgtgctt gttgtaggcg
      361 ctcagcacct tgtccagatt agcgtcggcc aggatcactc tcttggagaa ctcgctgatc
      421 tgctcgatga tctcgtccag gtagtgtttg tgctgttcca caaacagctg tttctgctca
      481 ttatcctcgg gggagccctt cagcttctca tagtggctgg ccaggtacag gaagttcaca
      541 tatttggagg gcagggccag ttcgtttccc ttctgcagtt cgccggcaga ggccagcatt
      601 ctcttccggc cgttttccag ctcgaacagg gagtacttag gcagcttgat gatcaggtcc
      661 tttttcactt ctttgtagcc cttggcttcc agaaagtcga tgggattctt ctcgaagctg
      721 cttctttcca tgatggtgat ccccagcagc tctttcacac tcttcagttt cttggacttg
      781 cccttttcca ctttggccac caccagcaca gaataggcca cggtggggct gtcgaagccg
      841 ccgtacttct tagggtccca gtccttcttt ctggcgatca gcttgtcgct gttcctcttg
      901 gg
//
