LOCUS       sequence-69347-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector containing SFFV promoter, dCas9 that is 
            fused to 2x NLS and tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pHR-SFFV-dCas9-BFP
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aggccgcctc tctcggcctt ggtcagattg tcgaacttcc tctgggtaat cagcttggca
       61 ttcagcagct ggcgccagta gttcttcatc ttcttcacga cctcttcgga gggcacgttg
      121 tcgctcttgc cccggttctt gtcgctccga gtcagcactt tgttatcgat ggagtcgtcc
      181 ttcagaaagc tctgaggcac gatagcgtcc acatcgtagt cggacagccg gttgatgtcc
      241 agttcctggt ccacgtacat atcccgccca ttctgcaggt agtacaggta cagcttctcg
      301 ttctgcagct gggtgttttc cacggggtgt tctttcagga tctggctgcc cagctctttg
      361 atgccctctt cgatccgctt cattctctcg cggctgttct tctgtccctt ctgggtggtc
      421 tggttctctc tggccatttc gatcacgatg ttctcgggct tgtgccggcc catcactttc
      481 acgagctcgt ccaccacctt cactgtctgc aggatgccct tcttaatggc ggggctgccg
      541 gccagattgg caatgtgctc gtgcaggcta tcgccctggc cggacacctg ggctttctgg
      601 atgtcctctt taaaggtcag gctgtcgtcg tggatcagct gcatgaagtt tctgttggcg
      661 aagccgtcgg acttcaggaa atccaggatt gtcttgccgg actgcttgtc ccggatgccg
      721 ttgatcagct tccggctcag cctgccccag ccggtgtatc tccgccgctt cagctgcttc
      781 atcactttgt cgtcgaacag gtgggcatag gttttcagcc gttcctcgat catctctctg
      841 tcctcaaaca gtgtcagggt cagcacgata tcttc
//
