LOCUS       sequence-69348-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vivo expression of Cas9 or generation of Cas9 mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, 
            Montague TG, Zimmerman S, Richter C, Schier AF
  TITLE     Efficient mutagenesis by Cas9 protein-mediated oligonucleotide 
            insertion and large-scale assessment of single-guide RNAs.
  JOURNAL   PLoS One. 2014 May 29;9(5):e98186. doi: 
            10.1371/journal.pone.0098186. eCollection 2014.
  PUBMED    24873830
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-29"; volume: "9(5):e98186. doi"; pages: " 
            10.1371/journal.pone.0098186. eCollection 2014"
COMMENT     Sequence Label: pCS2-Cas9
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..63)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgtctggatc tacgtaatac gactcactat agttctagat tacaccttcc tcttcttctt
       61 ggggtcagcc ctgctgtctc caccgagctg agagaggtcg attcttgttt catagagccc
      121 cgtaattgac tgatgaatca gtgtggcgtc caggacctcc tttgtagagg tgtaccgctt
      181 tctgtctatg gtggtgtcga agtacttgaa ggctgcaggc gcgcccaagt tggtcagagt
      241 aaacaagtgg ataatgtttt ctgcctgctc cctgatgggc ttatccctgt gcttattgta
      301 agcagaaagc accttatcga ggttagcgtc ggcgaggatc actcttttgg agaattcgct
      361 tatttgctcg atgatctcat caaggtagtg tttgtgttgt tccacgaaca gctgcttctg
      421 ctcattatct tcgggagacc ctttgagctt ttcatagtgg ctggccagat acaagaaatt
      481 aacgtattta gagggcagtg ccagctcgtt acctttctgc agctcgcccg cactagcgag
      541 cattcgtttc cggccgtttt caagctcaaa gagagagtac ttgggaagct taatgatgag
      601 gtcttttttg acctctttat atcctttcgc ctcgagaaag tcgatggggt ttttttcgaa
      661 gcttgatcgc tccatgattg tgatgcccag cagttccttg acgcttttga gttttttaga
      721 cttccctttc tccactttgg ccacaaccag tacactgtaa gcgactgtag gagaatcgaa
      781 tccgccgtat ttcttggggt cccaatcttt ttt
//
