LOCUS       sequence-69350-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MSCV-based vector encodes human cyclin E mutated within N- and 
            C-terminal Cdc4 phosphodegrons .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Xu Y, Swartz KL, Siu KT, Bhattacharyya M, Minella AC
  TITLE     Fbw7-dependent cyclin E regulation ensures terminal maturation of 
            bone marrow erythroid cells by restraining oxidative metabolism.
  JOURNAL   Oncogene. 2013 Jul 22. doi: 10.1038/onc.2013.289.
  PUBMED    23873023
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2013 Jul 22. doi: 10.1038/onc.2013.289."
COMMENT     Sequence Label: MIGR1-cyclin E-AA
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             132..646
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    808..824
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(832..838,839..856,857..862))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 actctcggca tggacgagct gtacaagtaa tgaattaatt aagaattatc aagcttatcg
       61 ataccgtcga cctgcagcca agcttatcga taaaataaaa gattttattt agtctccaga
      121 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
      181 tttgcaaggc atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac
      241 agagagacag cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
      301 tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac
      361 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
      421 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
      481 agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc
      541 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
      601 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatgggt aacagtttct
      661 tgaagttgga gaacaacatt ctgagggtag gagtcgaata ttaagtaatc ctgactcaat
      721 tagccactgt tttgaatcca catactccaa tactcctgaa atagttcatt atggacagcg
      781 cagaaagagc tggggagaat tgtgaaattg ttatccgctc acaattccac acaacatacg
      841 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      901 tgcgttgcg
//
