LOCUS       sequence-69354-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS 
            controlled by TDH3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pTDH3-dCas9
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(7..25)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    66..569
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        606..710
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
       61 agacggatcg cttgcctgta acttacacgc gcctcgtatc ttttaatgat ggaataattt
      121 gggaatttac tctgtgttta tttattttta tgttttgtat ttggatttta gaaagtaaat
      181 aaagaaggta gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt
      241 caacaaaaag cgtactttac atatatattt attagacaag aaaagcagat taaatagata
      301 tacattcgat taacgataag taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta
      361 cacagacaag atgaaacaat tcggcattaa tacctgagag caggaagagc aagataaaag
      421 gtagtatttg ttggcgatcc ccctagagtc ttttacatct tcggaaaaca aaaactattt
      481 tttctttaat ttcttttttt actttctatt tttaatttat atatttatat taaaaaattt
      541 aaattataat tatttttata gcacgtgatg aaaaggaccc aggtggcact tttcggggaa
      601 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      661 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      721 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      781 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      841 aca
//
