LOCUS       sequence-69358-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and 
            Mxi1 domain controlled by TDH3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pTDH3-dCas9-Mxi1
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aggccgtttc tgacagagta aaattcttga gggaactttc accattatgg gaaatggttc
       61 aagaaggtat tgacttaaac tccatcaaat ggtcaggtca ttgagtgttt tttatttgtt
      121 gtattttttt ttttttagag aaaatcctcc aatatataaa ttaggaatca tagtttcatg
      181 attttctgtt acacctaact ttttgtgtgg tgccctcctc cttgtcaata ttaatgttaa
      241 agtgcaattc tttttcctta tcacgttgag ccattagtat caatttgctt acctgtattc
      301 ctttacatcc tcctttttct ccttcttgat aaatgtatgt agattgcgta tatagtttcg
      361 tctaccctat gaacatattc cattttgtaa tttcgtgtcg tttctattat gaatttcatt
      421 tataaagttt atgtacaaat atcataaaaa aagagaatct ttttaagcaa ggattttctt
      481 aacttcttcg gcgacagcat caccgacttc ggtggtactg ttggaaccac ctaaatcacc
      541 agttctgata cctgcatcca aaaccttttt aactgcatct tcaatggcct taccttcttc
      601 aggcaagttc aatgacaatt tcaacatcat tgcagcagac aagatagtgg cgatagggtt
      661 gaccttattc tttggcaaat ctggagcaga accgtggcat ggttcgtaca aaccaaatgc
      721 ggtgttcttg tctggcaaag aggccaagga cgcagatggc a
//
