LOCUS       sequence-69359-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Notch reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Hansson EM, Teixeira AI, Gustafsson MV, Dohda T, Chapman G, Meletis 
            K, Muhr J, Lendahl U
  TITLE     Recording Notch signaling in real time.
  JOURNAL   Dev Neurosci. 2006;28(1-2):118-27.
  PUBMED    16508309
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev 
            Neurosci."; date: "2006"; volume: "28(1-2)"; pages: "118-27"
COMMENT     Sequence Label: 12XCSL-d1EGFP
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(28..44)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(28..44)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(41..63)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    52..68
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(76..82,83..100,101..106))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    121..142
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(259..276)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(510..529)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggtaccgag ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
       61 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      121 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      181 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      241 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      301 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      361 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      421 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      481 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      541 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      601 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      661 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      721 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      781 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttctcttg
//
