LOCUS       sequence-69361-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc tagged Miro2 T18N dominant negative mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Fransson S, Ruusala A, Aspenstrom P
  TITLE     The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in 
            mitochondrial trafficking.
  JOURNAL   Biochem Biophys Res Commun. 2006 Jun 2;344(2):500-10. Epub 2006 Apr 
            3.
  PUBMED    16630562
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun."; date: "2006-06-2"; volume: "344(2)"; pages: 
            "500-10. Epub 2006 Apr 3"
COMMENT     Sequence Label: pRK5-myc-Miro2 T18N
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        28..407
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..611
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     562..582
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     608..632
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        842..860
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     842..859
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tgattacgaa ttaattcgag ctcgcccgac attgattatt gactagttat taatagtaat
       61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      601 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
      661 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
      721 cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc
      781 ccttggcttc gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac
      841 gatttaggtg acactataga ataacatcca ctttgccttt ctc
//
