LOCUS       sequence-69365-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc tagged Miro2 S430N dominant negative mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Fransson S, Ruusala A, Aspenstrom P
  TITLE     The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in 
            mitochondrial trafficking.
  JOURNAL   Biochem Biophys Res Commun. 2006 Jun 2;344(2):500-10. Epub 2006 Apr 
            3.
  PUBMED    16630562
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun."; date: "2006-06-2"; volume: "344(2)"; pages: 
            "500-10. Epub 2006 Apr 3"
COMMENT     Sequence Label: pRK5-myc-Miro2 S430N
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        78..435
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        78..269
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(101..121)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      286..421
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(455..472)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(455..471)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(464..486)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(771..790)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtctggatcg ggaattaatt cggcgcagca ccatggcctg aaataacctc tgaaagagga
       61 acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
      121 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      181 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
      241 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
      301 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
      361 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
      421 ctaggctttt gcaaaaagct gttaacagct tggcactggc cgtcgtttta caacgtcgtg
      481 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
      541 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
      601 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
      661 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      721 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      781 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      841 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
      901 gg
//
