LOCUS       sequence-69367-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAC-EsaR with a208g mutation in esaR .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Shong J, Huang YM, Bystroff C, Collins CH
  TITLE     Directed evolution of the quorum-sensing regulator EsaR for 
            increased signal sensitivity.
  JOURNAL   ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub
            2013 Feb 6.
  PUBMED    23363022
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb3006402";
            journalName: "ACS Chem Biol"; date: "2013-04-19- 19"; volume: "8"; 
            issue: "4"; pages: "789-95"
COMMENT     Sequence Label: pAC-EsaR-I70V
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     186..203
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        595..697
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(764..783)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gggtttcgcc accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc
       61 ctatggaaaa acggctttgc cgcggccctc tcacttccct gttaagtatc ttcctggcat
      121 cttccaggaa atctccgccc cgttcgtaag ccatttccgc tcgccgcagt cgaacgaccg
      181 agcgtagcga gtcagtgagc gaggaagcgg aatatatcct gtatcacata ttctgctgac
      241 gcaccggtgc agcctttttt ctcctgccac atgaagcact tcactgacac cctcatcagt
      301 gccaacatag taagccagta tacactccgc tagcgctgat gtccggcggt gcttttgccg
      361 ttacgcacca ccccgtcagt agctgaacag gagggacagc tgatagaaac agaagccact
      421 ggagcacctc aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccgacgc
      481 actttgcgcc gaataaatac ctgtgacgga agatcacttc gcagaataaa taaatcctgg
      541 tgtccctgtt gataccggga agccctgggc caacttttgg cgaaaatgag acgttgatcg
      601 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
      661 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
      721 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
      781 tgctcaatgt acctataacc agaccgttca gctggatatt a
//
