LOCUS       sequence-69368-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Target RNAs used to determine complementarity requirements for in 
            vitro tailing and degradation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Ameres SL, Horwich MD, Hung JH, Xu J, Ghildiyal M, Weng Z, Zamore PD
  TITLE     Target RNA-directed trimming and tailing of small silencing RNAs.
  JOURNAL   Science. 2010 Jun 18;328(5985):1534-9.
  PUBMED    20558712
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2010-06-18"; volume: "328(5985)"; pages: "1534-9"
COMMENT     Sequence Label: pAS-3 (mm20-21)
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     223..240
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(399..426)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(518..604)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     653..675
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     667..684
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     668..684
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    700..799
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
       61 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      121 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
      181 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
      241 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
      301 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
      361 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
      421 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
      481 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
      541 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
      601 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
      661 cgacgttgta aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga
      721 ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact
      781 ttgtacaaaa aagcaggctc cgcggccgcc cccttcacca gatctcgagc tcaagctt
//
