LOCUS       sequence-69369-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Target RNAs used to determine complementarity requirements for in 
            vitro tailing and degradation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Ameres SL, Horwich MD, Hung JH, Xu J, Ghildiyal M, Weng Z, Zamore PD
  TITLE     Target RNA-directed trimming and tailing of small silencing RNAs.
  JOURNAL   Science. 2010 Jun 18;328(5985):1534-9.
  PUBMED    20558712
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2010-06-18"; volume: "328(5985)"; pages: "1534-9"
COMMENT     Sequence Label: pAS-6 (mm17-21)
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(226..325)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(342..361)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(343..361)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(366..382)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(366..382)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(484..503)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(562..581)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatg ataacaacct
       61 actacctcat ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
      121 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
      181 acttgtttat tgcagcttat aatggttaca agggtgggcg cgccgaccca gctttcttgt
      241 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
      301 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
      361 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
      421 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
      481 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
      541 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
      601 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
      661 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
      721 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
      781 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
      841 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcatt
//
