LOCUS       sequence-69370-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Target RNAs used to determine complementarity requirements for in 
            vitro tailing and degradation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Ameres SL, Horwich MD, Hung JH, Xu J, Ghildiyal M, Weng Z, Zamore PD
  TITLE     Target RNA-directed trimming and tailing of small silencing RNAs.
  JOURNAL   Science. 2010 Jun 18;328(5985):1534-9.
  PUBMED    20558712
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2010-06-18"; volume: "328(5985)"; pages: "1534-9"
COMMENT     Sequence Label: pAS-7 (mm12-21)
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(119..631,632..634,635..637))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     312..331
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     571..592
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      634..643
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    651..707
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(728..827)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(843..859)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
       61 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
      121 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
      181 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
      241 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
      301 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
      361 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc
      421 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct
      481 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
      541 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
      601 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt ggatcccggg
      661 cccgcggtac cgtcgactgc agaattcgaa gcttgagctc gagatctggt gaagggggcg
      721 gccgcggagc ctgctttttt gtacaaagtt ggcattataa aaaagcattg ctcatcaatt
      781 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgggg cccgagctta
      841 agactggccg tcgttttac
//
