LOCUS       sequence-69381-e        4225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for generation of knockout of mycobacterial genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U, 
            Nandicoori VK
  TITLE     Development of a new generation of vectors for gene expression, gene
            replacement, and protein-protein interaction studies in 
            mycobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Mar;79(5):1718-29. doi: 
            10.1128/AEM.03695-12. Epub 2013 Jan 11.
  PUBMED    23315736
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.03695-12"; journalName: "Appl Environ Microbiol"; date:
            "2013-03"; volume: "79"; issue: "5"; pages: "1718-29"
COMMENT     Sequence Label: pSt-KO
FEATURES             Location/Qualifiers
     source          1..4225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      112..700
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     601..620
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccttcaccg ttaacactag ttaattaatc tagagcggcc gctgcagggc ccgggatcca
       61 tggctagttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      121 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      181 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
      241 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
      301 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
      361 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
      421 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
      481 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
      541 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      601 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      661 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
      721 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      781 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
      841 aacgaccgag cgcaacgcgt tacactgcat gcggttagga atacggttag ccatttgcct
      901 gcttttatat agttatatgg gattcacctt tatgttgata agaaataaaa gaaaatgcca
      961 ataggatatc ggcattttct tttgcgtttt tatttgttaa ctgttaattg tccttgttca
     1021 aggatgctgt ctttgacaac agatgttttc ttgcctttga tgttcagcag gaagcttggc
     1081 gcaaacgttg attgtttgtc tgcgtagaat cctctgtttg tcatatagct tgtaatcacg
     1141 acattgtttc ctttcgcttg aggtacagcg aagtgtgagt aagtaaaggt tacatcgtta
     1201 ggatcaagat ccatttttaa cacaaggcca gttttgttca gcggcttgta tgggccagtt
     1261 aaagaattag aaacataacc aagcatgtaa atatcgttag acgtaatgcc gtcaatcgtc
     1321 atttttgatc cgcgggagtc agtgaacagg taccatttgc cgttcatttt aaagacgttc
     1381 gcgcgttcaa tttcatctgt tactgtgtta gatgcaatca gcggtttcat cacttttttc
     1441 agtgtgtaat catcgtttag ctcaatcata ccgagagcgc cgtttgctaa ctcagccgtg
     1501 cgttttttat cgctttgcag aagtttttga ctttcttgac ggaagaatga tgtgcttttg
     1561 ccatagtatg ctttgttaaa taaagattct tcgccttggt agccatcttc agttccagtg
     1621 tttgcttcaa atactaagta tttgtggcct ttatcttcta cgtagtgagg atctctcagc
     1681 gtatggttgt cgcctgagct gtagttgcct tcatcgatga actgctgtac attttgatac
     1741 gtttttccgt caccgtcaaa gattgattta taatcctcta caccgttgat gttcaaagag
     1801 ctgtctgatg ctgatacgtt aacttgtgca gttgtcagtg tttgtttgcc gtaatgttta
     1861 ccggagaaat cagtgtagaa taaacggatt tttccgtcag atgtaaatgt ggctgaacct
     1921 gaccattctt gtgtttggtc ttttaggata gaatcatttg catcgaattt gtcgctgtct
     1981 ttaaagacgc ggccagcgtt tttccagctg tcaatagaag tttcgccgac tttttgatag
     2041 aacatgtaaa tcgatgtgtc atccgcattt ttaggatctc cggctaatgc aaagacgatg
     2101 tggtagccgt gatagtttgc gacagtgccg tcagcgtttt gtaatggcca gctgtcccaa
     2161 acgtccaggc cttttgcaga agagatattt ttaattgtgg acgaatcgaa ttcaggaact
     2221 tgatattttt catttttttg ctgttcaggg atttgcagca tatcatggcg tgtaatatgg
     2281 gaaatgccgt atgtttcctt atatggcttt tggttcgttt ctttcatatg cgcaaacgct
     2341 tgagttgcgc ctcctgccag cagtgcggta gtaaaggtta atactgttgc ttgttttgca
     2401 aactttttga tgttcatcgt tcatgtctcc ttttttatgt actgtgttag cggtctgctt
     2461 cttccagccc tcctgtttga agatggcaag ttagttacgc acaataaaaa aagacctaaa
     2521 atatgtaagg ggtgacgcca aagtatcgac ctcgagtcac cgggtgacgg caaccccgct
     2581 acttacgctg gccgggcgca gtgcccgcga cggacggctc aagttgtcct cgctgccact
     2641 cgctgcgacg acgggcctgg cctcaccgtc ccgacctacg actcaccggt cgcgagtgcc
     2701 aacgttattc ttagcactcg cctatgccga gtgcaagaag ccccgcaccg ggtcatgccc
     2761 ctcgttcgac cttgtcctcg gccctccgat ccgggtgagt atgttcggcc catgaccgcc
     2821 aacgacaaca agacccgtaa atggtcggcc gcagacgtcc ccgatcaaag cgggcgcgtc
     2881 gttgtggtca cgcatgcggt ggtaccttga tccggcatga gattatcaaa aaggatcttc
     2941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3061 tttcgttcat ccatagttgc ctgactcccc gacgtggccg accagcccgt catcgtcaac
     3121 gcctgaccgc ggtgcggaca ggccgtgtcg cgaccggccg tgcggaatta agccggcccg
     3181 taccctgtga atagaggtcc gctgtgacac aagaatccct gttacttctc gaccgtattg
     3241 attcggatga ttcctacgcg agcctgcgga acgaccagga gttctgggag ccgctggccc
     3301 gccgagccct ggaggagctc gggctgccgg tgccgccggt gctgcgggtg cccggcgaga
     3361 gcaccaaccc cgtactggtc ggcgagcccg acccggtgat caagctgttc ggcgagcact
     3421 ggtgcggtcc ggagagcctc gcgtcggagt cggaggcgta cgcggtcctg gcggacgccc
     3481 cggtgccggt gccccgcctc ctcggccgcg gcgagctgcg gcccggcacc ggagcctggc
     3541 cgtggcccta cctggtgatg agccggatga ccggcaccac ctggcggtcc gcgatggacg
     3601 gcacgaccga ccggaacgcg ctgctcgccc tggcccgcga actcggccgg gtgctcggcc
     3661 ggctgcacag ggtgccgctg accgggaaca ccgtgctcac cccccattcc gaggtcttcc
     3721 cggaactgct gcgggaacgc cgcgcggcga ccgtcgagga ccaccgcggg tggggctacc
     3781 tctcgccccg gctgctggac cgcctggagg actggctgcc ggacgtggac acgctgctgg
     3841 ccggccgcga accccggttc gtccacggcg acctgcacgg gaccaacatc ttcgtggacc
     3901 tggccgcgac cgaggtcacc gggatcgtcg acttcaccga cgtctatgcg ggagactccc
     3961 gctacagcct ggtgcaactg catctcaacg ccttccgggg cgaccgcgag atcctggccg
     4021 cgctgctcga cggggcgcag tggaagcgga ccgaggactt cgcccgcgaa ctgctcgcct
     4081 tcaccttcct gcacgacttc gaggtgttcg aggagacccc gctggatctc tccggcttca
     4141 ccgatccgga ggaactggcg cagttcctct gggggccgcc ggacaccgcc cccggcgcct
     4201 gacgcccccc ggatcagtgg ccgcc
//
