LOCUS       sequence-69385-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 
            transactivator domain in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein 
            LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford
            GE, Reddy TE, Gersbach CA
  TITLE     RNA-guided gene activation by CRISPR-Cas9-based transcription 
            factors.
  JOURNAL   Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600.
  PUBMED    23892895
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600."
COMMENT     Sequence Label: pcDNA-dCas9-VP64
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aacctccacg ttcagctttc gttaaattat caaacttacg ttgagtgatt aacttggcgt
       61 ttagaagttg tctccaatag tttttcatct ttttgactac ttcttcactt ggaacgttat
      121 ccgatttacc acgattttta tcagaacgcg ttaagacctt attgtctatt gaatcgtctt
      181 taaggaaact ttgtggaaca atggcatcga catcataatc acttaaacga ttaatatcta
      241 attcttggtc cacatacatg tctcttccat tttggagata atagagatag agcttttcat
      301 tttgcaattg agtattttca acaggatgct ctttaagaat ctgacttcct aattctttga
      361 taccttcttc gattcgtttc atacgctctc gcgaattttt ctggcccttt tgagttgtct
      421 gattttcacg tgccatttca ataacgatat tttctggctt atgccgcccc attactttga
      481 ccaattcatc aacaactttt acagtctgta aaataccttt tttaatagca gggctaccag
      541 ctaaatttgc aatatgttca tgtaaactat cgccttgtcc agacacttgt gctttttgaa
      601 tgtcttcttt aaatgtcaaa ctatcatcat ggatcagctg cataaaattg cgattggcaa
      661 aaccatctga tttcaaaaaa tctaatattg ttttgccaga ttgcttatcc ctaataccat
      721 taatcaattt tcgagacaaa cgtccccaac cagtataacg gcgacgttta agctgtttca
      781 tcaccttatc atcaaagagg tgagcatatg ttttaagtct ttcctcaatc atctccctat
      841 cttcaaataa ggtc
//
