LOCUS       sequence-69387-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 
            transactivator domain in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein 
            LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford
            GE, Reddy TE, Gersbach CA
  TITLE     RNA-guided gene activation by CRISPR-Cas9-based transcription 
            factors.
  JOURNAL   Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600.
  PUBMED    23892895
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600."
COMMENT     Sequence Label: pcDNA-dCas9-VP64
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             91..156
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             163..183
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgccatg gactacaaag accatgacgg tgattataaa
      121 gatcatgaca tcgattacaa ggatgacgat gacaagatgg cccccaagaa gaagaggaag
      181 gtgggccgcg gaatggataa gaaatactca ataggcttag ctatcggcac aaatagcgtc
      241 ggatgggcgg tgatcactga tgaatataag gttccgtcta aaaagttcaa ggttctggga
      301 aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt tgacagtgga
      361 gagacagcgg aagcgactcg tctcaaacgg acagctcgta gaaggtatac acgtcggaag
      421 aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt
      481 ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat
      541 cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat
      601 catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt aatctatttg
      661 gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga tttaaatcct
      721 gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa tcaattattt
      781 gaagaa
//
