LOCUS       sequence-69392-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for TALE proteins fused to VP64 activation domain
            compatible with GoldenGate cloning system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Perez-Pinera P, Ousterout DG, Brunger JM, Farin AM, Glass KA, Guilak
            F, Crawford GE, Hartemink AJ, Gersbach CA
  TITLE     Synergistic and tunable human gene activation by combinations of 
            synthetic transcription factors.
  JOURNAL   Nat Methods. 2013 Mar;10(3):239-42. doi: 10.1038/nmeth.2361. Epub 
            2013 Feb 3.
  PUBMED    23377379
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2361"; journalName: "Nat Methods"; date: "2013-03"; 
            volume: "10"; issue: "3"; pages: "239-42"
COMMENT     Sequence Label: pcDNA3.1-GoldenGate-VP64
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             91..156
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             163..183
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     753..775
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     767..784
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     768..784
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgccatg gactacaaag accatgacgg tgattataaa
      121 gatcatgaca tcgattacaa ggatgacgat gacaagatgg cccccaagaa gaagaggaag
      181 gtgggccgcg gatctgtgga tctacgcacg ctcggctaca gtcagcagca gcaagagaag
      241 atcaaaccga aggtgcgttc gacagtggcg cagcaccacg aggcactggt gggccatggg
      301 tttacacacg cgcacatcgt tgcgctcagc caacacccgg cagcgttagg gaccgtcgct
      361 gtcacgtatc agcacataat cacggcgttg ccagaggcga cacacgaaga catcgttggc
      421 gtcggcaaac agtggtccgg cgcacgcgcc ctggaggcct tgctcacgga tgcgggggag
      481 ttgagaggtc cgccgttaca gttggacaca ggccaacttg tgaagattgc aaaacgtggc
      541 ggcgtgaccg caatggaggc agtgcatgca tcgcgcaatg cactgacggg tgcccccctg
      601 gagacgggcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
      661 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      721 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      781 cagtgagcgc gcgtaatacg ac
//
