LOCUS       sequence-69393-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for TALE proteins compatible with GoldenGate 
            cloning system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Perez-Pinera P, Ousterout DG, Brunger JM, Farin AM, Glass KA, Guilak
            F, Crawford GE, Hartemink AJ, Gersbach CA
  TITLE     Synergistic and tunable human gene activation by combinations of 
            synthetic transcription factors.
  JOURNAL   Nat Methods. 2013 Mar;10(3):239-42. doi: 10.1038/nmeth.2361. Epub 
            2013 Feb 3.
  PUBMED    23377379
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2361"; journalName: "Nat Methods"; date: "2013-03"; 
            volume: "10"; issue: "3"; pages: "239-42"
COMMENT     Sequence Label: pcDNA3.1-GoldenGate
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             90..155
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             162..182
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgccatgg actacaaaga ccatgacggt gattataaag
      121 atcatgacat cgattacaag gatgacgatg acaagatggc ccccaagaag aagaggaagg
      181 tgggccgcgg atctgtggat ctacgcacgc tcggctacag tcagcagcag caagagaaga
      241 tcaaaccgaa ggtgcgttcg acagtggcgc agcaccacga ggcactggtg ggccatgggt
      301 ttacacacgc gcacatcgtt gcgctcagcc aacacccggc agcgttaggg accgtcgctg
      361 tcacgtatca gcacataatc acggcgttgc cagaggcgac acacgaagac atcgttggcg
      421 tcggcaaaca gtggtccggc gcacgcgccc tggaggcctt gctcacggat gcgggggagt
      481 tgagaggtcc gccgttacag ttggacacag gccaacttgt gaagattgca aaacgtggcg
      541 gcgtgaccgc aatggaggca gtgcatgcat cgcgcaatgc actgacgggt gcccccctgg
      601 agacgggcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
      661 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
      721 aggcgattaa gttgggtaac gccagggtt
//
