LOCUS       sequence-69395-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for TALE proteins compatible with GoldenGate 
            cloning system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Perez-Pinera P, Ousterout DG, Brunger JM, Farin AM, Glass KA, Guilak
            F, Crawford GE, Hartemink AJ, Gersbach CA
  TITLE     Synergistic and tunable human gene activation by combinations of 
            synthetic transcription factors.
  JOURNAL   Nat Methods. 2013 Mar;10(3):239-42. doi: 10.1038/nmeth.2361. Epub 
            2013 Feb 3.
  PUBMED    23377379
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2361"; journalName: "Nat Methods"; date: "2013-03"; 
            volume: "10"; issue: "3"; pages: "239-42"
COMMENT     Sequence Label: pcDNA3.1-GoldenGate
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..50)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(63..83)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    278..299
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(314..319,320..337,338..344)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    352..368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     357..379
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     376..392
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     376..392
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             388..789
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQ"
     primer_bind     411..431
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        413..431
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(478..497)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(479..497)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(507..524)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(507..523)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(516..538)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cgcgaattcc taccgattca agaagcgtag tccggaacgt cgtacgggta gttaattaac
       61 ccaactttgc gtttcttttt cgggctagcc ttggatccgg caacgcgatg ggacgtgcgt
      121 tcgccaatac ggcgattgac tcttctgatc aattccggcg cgtgcggcaa tccctttttc
      181 actgcatcca tggcaggacg tccgccgagg caggccaagg cgacgaggtg gtcgttggag
      241 acgaccggtg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      301 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      361 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
      421 ctcactaaag ggaacaaaag ctggaggacc tcgagggggg gcccggtacc caattcgccc
      481 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
      541 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      601 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      661 tgggacgcgc cctgtagcgg cgcccgtctc caggggggca cccgtcagtg cattgcgcga
      721 tgcatgcact gcctccattg cggtcacgcc gccacgtttt gcaatcttca caagttggcc
      781 tgtgtccaac
//
