LOCUS       sequence-69396-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52
            promoter, targeting endogenous TEF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pSNR52-sgTEF1
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        3..218
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 ttttcaattc atcatttttt ttttattctt ttttttgatt tcggtttcct tgaaattttt
       61 ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg
      121 tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac
      181 tgcacagaac aaaaacgtgc aggaaacgaa gataaatcat gtcgaaagct acatataagg
      241 aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa
      301 agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag
      361 ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt
      421 tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac
      481 tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg
      541 gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag
      601 gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt
      661 tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta
      721 ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca
      781 tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg
      841 acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacag
//
