LOCUS       sequence-69397-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52
            promoter, targeting endogenous TRE elements of pTET07 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pSNR52-sgTET
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(19..37)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    78..581
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        618..722
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
       61 taatggtttc ttagacggat cgcttgcctg taacttacac gcgcctcgta tcttttaatg
      121 atggaataat ttgggaattt actctgtgtt tatttatttt tatgttttgt atttggattt
      181 tagaaagtaa ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa taaacaaagg
      241 tttaaaaaat ttcaacaaaa agcgtacttt acatatatat ttattagaca agaaaagcag
      301 attaaataga tatacattcg attaacgata agtaaaatgt aaaatcacag gattttcgtg
      361 tgtggtcttc tacacagaca agatgaaaca attcggcatt aatacctgag agcaggaaga
      421 gcaagataaa aggtagtatt tgttggcgat ccccctagag tcttttacat cttcggaaaa
      481 caaaaactat tttttcttta atttcttttt ttactttcta tttttaattt atatatttat
      541 attaaaaaat ttaaattata attattttta tagcacgtga tgaaaaggac ccaggtggca
      601 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      661 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      721 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
      781 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
      841 cacgagtggg ttacatcga
//
