LOCUS       sequence-69399-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for plasmid integration using the Flp-In system. AID at C-terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Holland AJ, Fachinetti D, Han JS, Cleveland DW
  TITLE     Inducible, reversible system for the rapid and complete degradation 
            of proteins in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 
            10.1073/pnas.1216880109. Epub 2012 Nov 12.
  PUBMED    23150568
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1216880109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-12-4- 4"; volume: "109"; issue: "49"; pages: "E3350-7"
COMMENT     Sequence Label: pcDNA5-H2B-AID-EYFP
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..459
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             655..858
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag cgtttaaact
       61 taagcttacc gtgccgccac catgccagag ccagcgaagt ctgctcccgc cccgaaaaag
      121 ggctccaaga aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg caagcgcagc
      181 cgcaaggaga gctattccat ctatgtgtac aaggttctga agcaggtcca ccctgacacc
      241 ggcatttcgt ccaaggccat gggcatcatg aattcgtttg tgaacgacat tttcgagcgc
      301 atcgcaggtg aggcttcccg cctggcgcat tacaacaagc gctcgaccat cacctccagg
      361 gagatccaga cggccgtgcg cctgctgctg cctggggagt tggccaagca cgccgtgtcc
      421 gagggtacta aggccatcac caagtacacc agcgctaagc ccgggatggg cagtgtcgag
      481 ctgaatctga gggagactga gctgtgtctt ggtcttcccg gtggagatac agtggctccg
      541 gtaaccggaa acaagagagg gttctcagag acggttgatc tgaagctaaa tctgaataat
      601 gagcctgcaa acaaggaagg atctacgact catgacgtcg tgacttttga ttccaaggag
      661 aagagtgctt gtcctaaaga tccagccaaa cctccggcca aggcacaagt tgtgggatgg
      721 ccaccggtga gatcataccg gaagaacgtg atggtttcct gccaaaaatc aagcggtggc
      781 ccggaggcgg cggcgttcgt gaaggtatca atggacggag caccgtactt gaggaaaatc
      841 gatttgagga tgtataaa
//
