LOCUS       sequence-69400-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein 
            LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford
            GE, Reddy TE, Gersbach CA
  TITLE     RNA-guided gene activation by CRISPR-Cas9-based transcription 
            factors.
  JOURNAL   Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600.
  PUBMED    23892895
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600."
COMMENT     Sequence Label: pcDNA-dCas9
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..28)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atcagcccac cttcctcttc ttcttggggt cacctcctag ctgactcaaa tcaatgcgtg
       61 tttcataaag accagtgatg gattgatgga taagagtggc atctaaaact tcttttgtag
      121 acgtatatcg tttacgatca attgttgtat caaaatattt aaaagcagcg ggagctccaa
      181 gattcgtcaa cgtaaataaa tgaataatat tttctgcttg ttcacgtatt ggtttgtctc
      241 tatgtttgtt atatgcacta agaactttat ctaaattggc atctgctaaa ataacacgct
      301 tagaaaattc actgatttgc tcaataatct catctaaata atgcttatgc tgctccacaa
      361 acaattgttt ttgttcgtta tcttctggac tacccttcaa cttttcataa tgactagcta
      421 aatataaaaa attcacatat ttgcttggca gagccagctc atttcctttt tgtaattctc
      481 cggcactagc cagcatccgt ttacgaccgt tttctaactc aaaaagacta tatttaggta
      541 gtttaatgat taagtctttt ttaacttcct tatatccttt agcttctaaa aagtcaatcg
      601 gatttttttc aaaggaactt ctttccataa ttgtgatccc tagtaactct ttaacggatt
      661 ttaacttctt cgatttccct ttttccacct tagcaaccac taggactgaa taagctaccg
      721 ttggactatc aaaaccacca tatttttttg gatcccagtc ttttttacga gcaataagct
      781 tgtccgaatt tc
//
