LOCUS       sequence-69401-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 
            transactivator domain in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein 
            LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford
            GE, Reddy TE, Gersbach CA
  TITLE     RNA-guided gene activation by CRISPR-Cas9-based transcription 
            factors.
  JOURNAL   Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600.
  PUBMED    23892895
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600."
COMMENT     Sequence Label: pcDNA-dCas9-VP64
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..55)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(62..211)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(221..241)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 acggccgcga attcctaccg attcaagaag cgtagtccgg aacgtcgtac gggtagttaa
       61 ttaacatatc gagatcgaaa tcgtccagag catcggagcc gagcatgtcc agatcaaagt
      121 catccaatgc gtcgcttccc aacatatcca ggtcaaagtc atcgagggca tcagaaccca
      181 gcatgtcgag atcgaaatcg tccagcgcgt cggcgcgccc aactttgcgt ttctttttcg
      241 ggctagcctt ggatccggca attgggtcac ctcctagctg actcaaatca atgcgtgttt
      301 cataaagacc agtgatggat tgatggataa gagtggcatc taaaacttct tttgtagacg
      361 tatatcgttt acgatcaatt gttgtatcaa aatatttaaa agcagcggga gctccaagat
      421 tcgtcaacgt aaataaatga ataatatttt ctgcttgttc acgtattggt ttgtctctat
      481 gtttgttata tgcactaaga actttatcta aattggcatc tgctaaaata acacgcttag
      541 aaaattcact gatttgctca ataatctcat ctaaataatg cttatgctgc tccacaaaca
      601 attgtttttg ttcgttatct tctggactac ccttcaactt ttcataatga ctagctaaat
      661 ataaaaaatt cacatatttg cttggcagag ccagctcatt tcctttttgt aattctccgg
      721 cactagccag catccgttta cgaccgtttt ctaactcaaa aagactatat ttaggtagtt
      781 taatgattaa gtct
//
