LOCUS       sequence-69402-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for plasmid integration using the Flp-In system. AID at C-terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Holland AJ, Fachinetti D, Han JS, Cleveland DW
  TITLE     Inducible, reversible system for the rapid and complete degradation 
            of proteins in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 
            10.1073/pnas.1216880109. Epub 2012 Nov 12.
  PUBMED    23150568
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1216880109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-12-4- 4"; volume: "109"; issue: "49"; pages: "E3350-7"
COMMENT     Sequence Label: pcDNA5-H2B-AID-EYFP
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..708)
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNN
                     EPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSG
                     GPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMN
                     ERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAME
                     KCKSRA"
ORIGIN
        1 ctcgcccttg ctcactggat ccgcagctct gctcttgcac ttctccatcg ccctcggagc
       61 gagaccaatg gcatccgatc ctttcatgag acgtaaacgc ttgcatgtat cgacgaacat
      121 tggccaagga acgtcgccga cgagcatcca atcaccgtct ttgtcttcat aagagggaac
      181 atagtcccag ctattcacca aatccatcaa tttcctctca ttcatgaagt ctatcattcc
      241 ttcttctcct ccatgtttgc ccatggtaaa agagctgaac atgttggaca aagcattaga
      301 aagctcatcg tagcttttat acatcctcaa atcgattttc ctcaagtacg gtgctccgtc
      361 cattgatacc ttcacgaacg ccgccgcctc cgggccaccg cttgattttt ggcaggaaac
      421 catcacgttc ttccggtatg atctcaccgg tggccatccc acaacttgtg ccttggccgg
      481 aggtttggct ggatctttag gacaagcact cttctccttg gaatcaaaag tcacgacgtc
      541 atgagtcgta gatccttcct tgtttgcagg ctcattattc agatttagct tcagatcaac
      601 cgtctctgag aaccctctct tgtttccggt taccggagcc actgtatctc caccgggaag
      661 accaagacac agctcagtct ccctcagatt cagctcgaca ctgcccatcc cgggcttagc
      721 gctggtgtac ttggtgatgg ccttagtacc ctcggacacg gcgtgcttgg ccaactcccc
      781 aggcagcagc aggcgcacgg ccgtctggat ctccctggag gtgatggtcg agcgcttgtt
      841 gtaatgcgcc aggcgggaag cctcac
//
