LOCUS       sequence-69403-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for plasmid integration using the Flp-In system. AID at N-terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Holland AJ, Fachinetti D, Han JS, Cleveland DW
  TITLE     Inducible, reversible system for the rapid and complete degradation 
            of proteins in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 
            10.1073/pnas.1216880109. Epub 2012 Nov 12.
  PUBMED    23150568
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1216880109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-12-4- 4"; volume: "109"; issue: "49"; pages: "E3350-7"
COMMENT     Sequence Label: pcDNA5-EGFP-AID-BubR1
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             238..267
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(271..273,274..786)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(313..334)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(574..593)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag cgtttaaact
       61 taagcttggt accccgggga attcggcaga tcccgaggtc cgacagcgcc cggcccagat
      121 ccccacgcct gccaggagca agccgagagc cagccggccg gcgcactccg actccgagca
      181 gtctctgtcc ttcgacccga gccccgcgcc ctttccggga cccctgcccc gcggatggag
      241 cagaagctca tctcagaaga agacctcgac gtgagcaagg gcgaggagct gttcaccggg
      301 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      361 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      421 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      481 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      541 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      601 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      661 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      721 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
      781 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      841 g
//
