LOCUS       sequence-69405-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for plasmid integration using the Flp-In system. AID at N-terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Holland AJ, Fachinetti D, Han JS, Cleveland DW
  TITLE     Inducible, reversible system for the rapid and complete degradation 
            of proteins in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 
            10.1073/pnas.1216880109. Epub 2012 Nov 12.
  PUBMED    23150568
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1216880109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-12-4- 4"; volume: "109"; issue: "49"; pages: "E3350-7"
COMMENT     Sequence Label: pcDNA5-EGFP-AID-BubR1
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..705
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNN
                     EPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSG
                     GPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMN
                     ERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAME
                     KCKSRA"
ORIGIN
        1 gacgagctgt acaaggcgga tatgggcagt gtcgagctga atctgaggga gactgagctg
       61 tgtcttggtc ttcccggtgg agatacagtg gctccggtaa ccggaaacaa gagagggttc
      121 tcagagacgg ttgatctgaa gctaaatctg aataatgagc ctgcaaacaa ggaaggatct
      181 acgactcatg acgtcgtgac ttttgattcc aaggagaaga gtgcttgtcc taaagatcca
      241 gccaaacctc cggccaaggc acaagttgtg ggatggccac cggtgagatc ataccggaag
      301 aacgtgatgg tttcctgcca aaaatcaagc ggtggcccgg aggcggcggc gttcgtgaag
      361 gtatcaatgg acggagcacc gtacttgagg aaaatcgatt tgaggatgta taaaagctac
      421 gatgagcttt ctaatgcttt gtccaacatg ttcagctctt ttaccatggg caaacatgga
      481 ggagaagaag gaatgataga cttcatgaat gagaggaaat tgatggattt ggtgaatagc
      541 tgggactatg ttccctctta tgaagacaaa gacggtgatt ggatgctcgt cggcgacgtt
      601 ccttggccaa tgttcgtcga tacatgcaag cgtttacgtc tcatgaaagg atcggatgcc
      661 attggtctcg ctccgagggc gatggagaag tgcaagagca gagctggatc ccatatggcg
      721 gcggtgaaga aggaaggggg tgctctgagt gaagccatgt ccctggaggg agatgaatgg
      781 gaactgagta aagaaaatgt acaaccttta aggcaagggc ggatcatgtc cacgcttcag
      841 g
//
