LOCUS       sequence-69407-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For retroviral expression of E3 ubiquitin ligase osTIR1 with N-term 
            NES and C-term 9x myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Holland AJ, Fachinetti D, Han JS, Cleveland DW
  TITLE     Inducible, reversible system for the rapid and complete degradation 
            of proteins in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 
            10.1073/pnas.1216880109. Epub 2012 Nov 12.
  PUBMED    23150568
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1216880109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-12-4- 4"; volume: "109"; issue: "49"; pages: "E3350-7"
COMMENT     Sequence Label: pBabe TIR1-9myc
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tggtggtaca tcgccgccac catggcatta caaaaaaaat tagaagaatt agaattagat
       61 gaaggcggca cgtacttccc ggaggaggtg gtggagcaca tcttcagctt cctgccggcg
      121 cagcgcgacc gcaacacggt ctcgctcgtc tgcaaggtgt ggtacgagat cgagaggctg
      181 agccgccgcg gcgtcttcgt gggcaactgc tacgccgtgc gcgccggccg cgtcgccgcg
      241 cggttcccca acgtgcgggc gctcacggtg aaggggaagc cccacttcgc cgacttcaac
      301 ctcgtgcccc ccgactgggg cggctacgcg gggccgtgga tcgaggcggc cgcgagggga
      361 tgccacggcc tggaggagct caggatgaag cggatggtgg tgtccgacga gagcctcgag
      421 ctgctggctc gctcgttccc gcggttcagg gctcttgttc ttatcagctg cgaggggttc
      481 agcactgacg ggctagccgc cgtcgcgagc cattgcaagc ttctgaggga gttggatttg
      541 caggaaaatg aagtggagga tcgagggcct aggtggcttt cctgcttccc tgattcctgc
      601 acatcacttg tctcattgaa ttttgcctgc atcaaagggg aggttaatgc tggttcactg
      661 gagagacttg ttagcaggtc cccaaacctg cggagtttga ggctgaatcg atctgtatcg
      721 gtagatacac ttgcaaagat actactgcgt acccctaact tggaggattt ggggacaggg
      781 aatttgacag atgacttcca aactgagtcc tactttaagc ttaccatg
//
