LOCUS       sequence-69408-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For retroviral expression of E3 ubiquitin ligase osTIR1 with N-term 
            NES and C-term 9x myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Holland AJ, Fachinetti D, Han JS, Cleveland DW
  TITLE     Inducible, reversible system for the rapid and complete degradation 
            of proteins in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 
            10.1073/pnas.1216880109. Epub 2012 Nov 12.
  PUBMED    23150568
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1216880109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-12-4- 4"; volume: "109"; issue: "49"; pages: "E3350-7"
COMMENT     Sequence Label: pBabe TIR1-9myc
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..77)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(84..113)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(120..149)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(168..197)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(204..233)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(240..269)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(288..317)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(324..353)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(360..389)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctggcgaatt cgaatgatcg ttccactttt tagctagtgg atccgttcaa gtcttcttct
       61 gagattaatt tttgttcacc gttcaagtct tcctcggaga ttagcttttg ttcaccgttc
      121 aaatcttctt cagaaatcaa cttttgttca cctctagagg atccgttcaa gtcttcttct
      181 gagattaatt tttgttcacc gttcaagtct tcctcggaga ttagcttttg ttcaccgttc
      241 aaatcttctt cagaaatcaa cttttgttca cctctagagg atccgttcaa gtcttcttct
      301 gagattaatt tttgttcacc gttcaagtct tcctcggaga ttagcttttg ttcaccgttc
      361 aaatcttctt cagaaatcaa cttttgttca ccactagcag cagaaccgga gtcgactagg
      421 attttaacaa aatttggtgc atcatccctc gccccagcag ttgtgcggta cacatataat
      481 ttctccactt taggcagatc tccatggttt tcctccattt cattgctacc atcccgctca
      541 tttatgacct caacattgag catcggcatc tttgacgcaa ggacttggca cccctttaac
      601 gtgacattgc aagatgacat ccaaagggat cgcattgtct cgtacctagc aaaattcccc
      661 aagagtgcag catcaccaaa cgggctatct cttatctcca gtttccttaa attcttgcat
      721 ccattcataa catgcatcat acccttatca ctgtcaccag caaatgctat agaaagcatc
      781 tcaagttgtt ttgcatattt cccaatatac atgaaaactt tgtctgtgag aa
//
