LOCUS       sequence-69417-e         587 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid with EGFP expressed from synthetic 
            promoter (random mutagenesis of CMVwt 3' end)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Ferreira JP, Peacock RW, Lawhorn IE, Wang CL
  TITLE     Modulating ectopic gene expression levels by using retroviral 
            vectors equipped with synthetic promoters.
  JOURNAL   Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 
            10.1007/s11693-011-9089-0. Epub 2011 Nov 20.
  PUBMED    23205156
REFERENCE   2  (bases 1 to 587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Syst Synth 
            Biol."; date: "2011-12"; volume: "5(3-4):131-8. doi"; pages: " 
            10.1007/s11693-011-9089-0. Epub 2011 Nov 20"
COMMENT     Sequence Label: pCOCK CM-R05
FEATURES             Location/Qualifiers
     source          1..587
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        60..363
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tagtattaat agtaatcaat tacggggtca ttagttcgta gcccatatat ggagttccgc
       61 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      121 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      181 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      241 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      301 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      361 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      421 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      481 gactttccaa aatgtcgtaa caactccgtc ccattgacgc aacttggcgg caggggtaga
      541 catcagaggt ctagagcagc cgagctgata ctttgacctg acatatc
//
