LOCUS       sequence-69423-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Manning BD, Tee AR, Logsdon MN, Blenis J, Cantley LC
  TITLE     Identification of the tuberous sclerosis complex-2 tumor suppressor 
            gene product tuberin as a target of the phosphoinositide 
            3-kinase/akt pathway.
  JOURNAL   Mol Cell. 2002 Jul . 10(1):151-62.
  PUBMED    12150915
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2002 Jul . 10(1):151-62."
COMMENT     Sequence Label: pcDNA3 Flag TSC2
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             77..100
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttagcc accatggact acaaagacga tgacgataaa gcaagggatc cagccaaacc
      121 aacaagcaaa gattcaggct tgaaggagaa gtttaagatt ctgttgggac tgggaacacc
      181 gaggccaaat cccaggtctg cagagggtaa acagacggag tttatcatca ccgcggaaat
      241 actgagagaa ctgagcatgg aatgtggcct caacaatcgc atccggatga tagggcagat
      301 ttgtgaagtc gcaaaaacca agaaatttga agagcacgca gtggaagcac tctggaaggc
      361 ggtcgcggat ctgttgcagc cggagcggcc gctggaggcc cggcacgcgg tgctggctct
      421 gctgaaggcc atcgtgcagg ggcagggcga gcgtttgggg gtcctcagag ccctcttctt
      481 taaggtcatc aaggattacc cttccaacga agaccttcac gaaaggctgg aggttttcaa
      541 ggccctcaca gacaatggga gacacatcac ctacttggag gaagagctgg ctgactttgt
      601 cctgcagtgg atggatgttg gcttgtcctc ggaattcctt ctggtgctgg tgaacttggt
      661 caaattcaat agctgttacc tcgacgagta catcgcaagg atggttcaga tgatctgtct
      721 gctgtgcgtc cggaccgcgt cctctgtgga catagaggtc tccctgcagg tgctggacgc
      781 cgtggtctgc tacaactgcc tgccggctga gagcctcccg ctgttcatcg ttaccctctg
      841 tcgcaccatc aacgtcaagg agctctgcga gccttgctgg aagctgatgc
//
