LOCUS       sequence-69425-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vitro expression  and purification of Cas9 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, 
            Montague TG, Zimmerman S, Richter C, Schier AF
  TITLE     Efficient mutagenesis by Cas9 protein-mediated oligonucleotide 
            insertion and large-scale assessment of single-guide RNAs.
  JOURNAL   PLoS One. 2014 May 29;9(5):e98186. doi: 
            10.1371/journal.pone.0098186. eCollection 2014.
  PUBMED    24873830
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-29"; volume: "9(5):e98186. doi"; pages: " 
            10.1371/journal.pone.0098186. eCollection 2014"
COMMENT     Sequence Label: pET-28b-Cas9-His
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(68..88)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgccac cttcctcttc ttcttggggt cagccctgct gtctccaccg agctgagaga
      121 ggtcgattct tgtttcatag agccccgtaa ttgactgatg aatcagtgtg gcgtccagga
      181 cctcctttgt agaggtgtac cgctttctgt ctatggtggt gtcgaagtac ttgaaggctg
      241 caggcgcgcc caagttggtc agagtaaaca agtggataat gttttctgcc tgctccctga
      301 tgggcttatc cctgtgctta ttgtaagcag aaagcacctt atcgaggtta gcgtcggcga
      361 ggatcactct tttggagaat tcgcttattt gctcgatgat ctcatcaagg tagtgtttgt
      421 gttgttccac gaacagctgc ttctgctcat tatcttcggg agaccctttg agcttttcat
      481 agtggctggc cagatacaag aaattaacgt atttagaggg cagtgccagc tcgttacctt
      541 tctgcagctc gcccgcacta gcgagcattc gtttccggcc gttttcaagc tcaaagagag
      601 agtacttggg aagcttaatg atgaggtctt ttttgacctc tttatatcct ttcgcctcga
      661 gaaagtcgat ggggtttttt tcgaagcttg atcgctccat gattgtgatg cccagcagtt
      721 ccttgacgct tttgagtttt ttagacttcc ctttctccac tttggccaca accagtacac
      781 tgtaagcgac tgtaggagaa tcgaatccgc cgtatttctt ggggtcccaa tcttttttgc
      841 gtgcga
//
