LOCUS       sequence-69433-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8NmCitrine
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..21
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3..21
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    134..268
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(171..190)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     225..244
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(361..612)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(403..424)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     752..771
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcaattaacc ctcactaaag ggaacaaaag ctgggtaccg ggcccaatgc attggcgccg
       61 cggccgcgaa ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
      121 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
      181 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
      241 catcaatgta tcttatcatg tctggatcta cgtaatacga ctcactatag ttctagaggc
      301 tcgagaggcc ttggcgatcg ctggccggcc tttaattaat ggcgcgccgg ctccgaattc
      361 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
      421 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactggtagc tcaggtagtg
      481 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      541 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
      601 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
      661 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
      721 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
      781 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
      841 catgtggtcg gggtagcggg cgaagcacat caggccgta
//
