LOCUS       sequence-69434-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag, Myc tagged mutant Nbr in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Han BW, Hung JH, Weng Z, Zamore PD, Ameres SL
  TITLE     The 3'-to-5' exoribonuclease Nibbler shapes the 3' ends of microRNAs
            bound to Drosophila Argonaute1.
  JOURNAL   Curr Biol. 2011 Nov 22;21(22):1878-87. Epub 2011 Nov 3.
  PUBMED    22055293
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2011-11-22"; volume: "21(22)"; pages: "1878-87. Epub 
            2011 Nov 3"
COMMENT     Sequence Label: pAFMW-Nbr-D435A/E437A
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(448..468)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          598..663
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     protein_bind    755..779
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatct ctagtcaagg cactatacat caaatattcc ttattaaccc ctttacaaat
      181 taaaaagcta aaggtacaca atttttgagc atagttatta atagcagaca ctctatgcct
      241 gtgtggagta agaaaaaaca gtatgttatg attataactg ttatgcctac ttataaaggt
      301 tacagaatat ttttccataa ttttcttgta tagcagtgca gctttttcct ttgtggtgta
      361 aatagcaaag caagcaagag ttctattact aaacacagca tgactcaaaa aacttagcaa
      421 ttctgaagga aagtccttgg ggtcttctac ctttctcttc ttttttggag gagtagaatg
      481 ttgagagtca gcagtagcct catcatcact agatggcatt tcttctgagc aaaacaggtt
      541 ttcctcatta aaggcattcc accactgctc ccattcatca gttccatagg ttggaatcta
      601 aaatacacaa acaattagaa tcagtagttt aacacattat acacttaaaa attttatatt
      661 taccttagag ctttaaatct ctgtaggtag tttgtccaat tatgtcacac cacagaagta
      721 aggttccttc acaaagatcc tgctagctta cgtcaccact ttgtacaaga aagctgggtc
      781 ggcgcgccca ccctttcact taacatgggc accccgcctg agaaagttat tgctggcgat
      841 gctcttttcg ataaccgctt gtatg
//
