LOCUS       sequence-69446-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bmal fragment cloned with Venus tag and used for BiFC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Huang N, Chelliah Y, Shan Y, Taylor CA, Yoo SH, Partch C, Green CB, 
            Zhang H, Takahashi JS
  TITLE     Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional 
            activator complex.
  JOURNAL   Science. 2012 Jul 13;337(6091):189-94. doi: 10.1126/science.1222804.
            Epub 2012 May 31.
  PUBMED    22653727
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1222804"; journalName: "Science"; date: 
            "2012-07-13- 13"; volume: "337"; issue: "6091"; pages: "189-94"
COMMENT     Sequence Label: HLH Bmal Pas 1-465-VenC
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(150..401)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(192..213)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      404..413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcag ttatctagat
      121 ccggtggatc ccgagggcag cattccggac ttgtacagct cgtccatgcc gagagtgatc
      181 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
      241 ctcagggcgg actggtagct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
      301 atgggggtgt tctgctggta gtggtcggcg agctgcacgc cgccgtcctc gatgttgtgg
      361 cggatcttga agttggcctt gatgccgttc ttctgcttgt ccaccatggt ggcgaccggg
      421 ctgtccatgc tgtggggggg tgctgttagc tgcgggaagg ttgggtcccc gccttccagg
      481 acattggcta aaacaacagt gttggttgag acaatgtatt caacttcctt ggtccacggg
      541 ttcatgaaac tgaaccatcg acttcgtagc gtgataaaag aaccatcttt gatcttaaac
      601 ttatagcaat tagttgtgat cttttctctt gtctgtaaaa cttgcctgtg acattctgcg
      661 aggtgtccta tgtcgtcttg atgaaaatac tcataacatg atgtacctag aagttcctgt
      721 ggtagatacg ccaaaatagc tgtcgccctc tgatctacaa aaacaaattt cccatctatt
      781 gcgtgtcgag aaacgtactc catagatttc acccgtattt ccccgttcgc tggttgtgga
      841 accatgtgcg a
//
