LOCUS       sequence-69449-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vivo expression of Cas9 or generation of Cas9 mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, 
            Montague TG, Zimmerman S, Richter C, Schier AF
  TITLE     Efficient mutagenesis by Cas9 protein-mediated oligonucleotide 
            insertion and large-scale assessment of single-guide RNAs.
  JOURNAL   PLoS One. 2014 May 29;9(5):e98186. doi: 
            10.1371/journal.pone.0098186. eCollection 2014.
  PUBMED    24873830
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-29"; volume: "9(5):e98186. doi"; pages: " 
            10.1371/journal.pone.0098186. eCollection 2014"
COMMENT     Sequence Label: pCS2-Cas9
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggatccatcg atgccaccat ggacaagaag tactccattg ggctcgatat cggcacaaac
       61 agcgtcggct gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa attcaaagtt
      121 ctgggcaata ccgatcgcca cagcataaag aagaacctca ttggcgccct cctgttcgac
      181 tccggggaga cggccgaagc cacgcggctc aaaagaacag cacggcgcag atatacccgc
      241 agaaagaatc ggatctgcta cctgcaggag atctttagta atgagatggc taaggtggat
      301 gactctttct tccataggct ggaggagtcc tttttggtgg aggaggataa aaagcacgag
      361 cgccacccaa tctttggcaa tatcgtggac gaggtggcgt accatgaaaa gtacccaacc
      421 atatatcatc tgaggaagaa gcttgtagac agtactgata aggctgactt gcggttgatc
      481 tatctcgcgc tggcgcatat gatcaaattt cggggacact tcctcatcga gggggacctg
      541 aacccagaca acagcgatgt cgacaaactc tttatccaac tggttcagac ttacaatcag
      601 cttttcgaag agaacccgat caacgcatcc ggagttgacg ccaaagcaat cctgagcgct
      661 aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac agctccctgg ggagaagaag
      721 aacggcctgt ttggtaatct tatcgccctg tcactcgggc tgacccccaa ctttaaatct
      781 aacttcgacc tggccgaaga tgccaagctt caactgagca aagacaccta cgatgatgat
      841 ctcgacaatc tgctggccca gatcggcgac cagtacgca
//
