LOCUS       sequence-69470-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bmal fragment cloned with Venus tag used for mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Huang N, Chelliah Y, Shan Y, Taylor CA, Yoo SH, Partch C, Green CB, 
            Zhang H, Takahashi JS
  TITLE     Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional 
            activator complex.
  JOURNAL   Science. 2012 Jul 13;337(6091):189-94. doi: 10.1126/science.1222804.
            Epub 2012 May 31.
  PUBMED    22653727
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1222804"; journalName: "Science"; date: 
            "2012-07-13- 13"; volume: "337"; issue: "6091"; pages: "189-94"
COMMENT     Sequence Label: HLH Bmal Pas 1-465-VenC L95E/L150E/W427A
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(152..403)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(194..215)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      406..415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag
      121 atccggtgga tcccgagggc agcattccgg acttgtacag ctcgtccatg ccgagagtga
      181 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
      241 tgctcagggc ggactggtag ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
      301 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gccgccgtcc tcgatgttgt
      361 ggcggatctt gaagttggcc ttgatgccgt tcttctgctt gtccaccatg gtggcgaccg
      421 ggctgtccat gctgtggggg ggtgctgtta gctgcgggaa ggttgggtcc ccgccttcca
      481 ggacattggc taaaacaaca gtgttggttg agacaatgta ttcaacttcc ttggtcgccg
      541 ggttcatgaa actgaaccat cgacttcgta gcgtgataaa agaaccatct ttgatcttaa
      601 acttatagca attagttgtg atcttttctc ttgtctgtaa aacttgcctg tgacattctg
      661 cgaggtgtcc tatgtcgtct tgatgaaaat actcataaca tgatgtacct agaagttcct
      721 gtggtagata cgccaaaata gctgtcgccc tctgatctac aaaaacaaat ttcccatcta
      781 ttgc
//
