LOCUS       sequence-69472-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bmal fragment cloned with Venus tag used for mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Huang N, Chelliah Y, Shan Y, Taylor CA, Yoo SH, Partch C, Green CB, 
            Zhang H, Takahashi JS
  TITLE     Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional 
            activator complex.
  JOURNAL   Science. 2012 Jul 13;337(6091):189-94. doi: 10.1126/science.1222804.
            Epub 2012 May 31.
  PUBMED    22653727
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1222804"; journalName: "Science"; date: 
            "2012-07-13- 13"; volume: "337"; issue: "6091"; pages: "189-94"
COMMENT     Sequence Label: HLH Bmal Pas 1-465-VenC L115E
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(151..402)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(193..214)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      405..414
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca gttatctaga
      121 tccggtggat cccgagggca gcattccgga cttgtacagc tcgtccatgc cgagagtgat
      181 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      241 gctcagggcg gactggtagc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
      301 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ccgccgtcct cgatgttgtg
      361 gcggatcttg aagttggcct tgatgccgtt cttctgcttg tccaccatgg tggcgaccgg
      421 gctgtccatg ctgtgggggg gtgctgttag ctgcgggaag gttgggtccc cgccttccag
      481 gacattggct aaaacaacag tgttggttga gacaatgtat tcaacttcct tggtccacgg
      541 gttcatgaaa ctgaaccatc gacttcgtag cgtgataaaa gaaccatctt tgatcttaaa
      601 cttatagcaa ttagttgtga tcttttctct tgtctgtaaa acttgcctgt gacattctgc
      661 gaggtgtcct atgtcgtctt gatgaaaata ctcataacat gatgtaccta gaagttcctg
      721 tggtagatac gccaaaatag ctgtcgccct ctgatctaca aaaacaaatt tcccatctat
      781 tgcgtgtc
//
