LOCUS       sequence-69473-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is a Gateway destination vector for secreted expression in 
            Drosophila cell culture by induction via CuSO4. Inserts will be 
            C-terminally tagged with Fc, V5 and hexahistidine tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Ozkan E, Carrillo RA, Eastman CL, Weiszmann R, Waghray D, Johnson 
            KG, Zinn K, Celniker SE, Garcia KC
  TITLE     An Extracellular Interactome of Immunoglobulin and LRR Proteins 
            Reveals Receptor-Ligand Networks.
  JOURNAL   Cell. 2013 Jul 3;154(1):228-39. doi: 10.1016/j.cell.2013.06.006.
  PUBMED    23827685
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "228-39"
COMMENT     Sequence Label: pECIA2
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    126..260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(163..182)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     217..236
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     293..310
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(333..350)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(360..401)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gcatgcctgc aggtcgactg atcataatca gccataccac atttgtagag gttttacttg
       61 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
      121 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
      181 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
      241 tatcttatca tgtctggatc ctcgatcgag gctgatcagc gagctcaggc cttagaaggc
      301 acagtcgagg ctgatcagcg ggtttaaact caatggtgat ggtgatgatg accggtacgc
      361 gtagaatcga gaccgaggag agggttaggg ataggcttac cttcgaaggg ccctctagat
      421 ttacccggag acagggagag gctcttctgc gtgtagtggt tgtgcagacc ctcatgcatc
      481 acggagcatg agaagacgtt cccctgctgc cacctgctct tgtccacggt gagcttgctg
      541 tagaggaaga aggagccgtc ggagtccagc acgggaggcg tggtcttgta gttgttctcc
      601 ggctgcccat tgctctccca ctccacggcg atgtcgctgg gatagaagcc tttgaccagg
      661 caggtcaggc tgacctggtt cttggtcatc tcctcccggg atgggggcag ggtgtacacc
      721 tgtggttctc ggggctgccc tttggctttg gagatggttt tctcgatggg
//
