LOCUS       sequence-69474-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is a Gateway destination vector for secreted expression in 
            Drosophila cell culture by CuSO4 induction. Inserts will be 
            pentamerized and tagged with Alkaline phosphatase, FLAG and His 
            tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Ozkan E, Carrillo RA, Eastman CL, Weiszmann R, Waghray D, Johnson 
            KG, Zinn K, Celniker SE, Garcia KC
  TITLE     An Extracellular Interactome of Immunoglobulin and LRR Proteins 
            Reveals Receptor-Ligand Networks.
  JOURNAL   Cell. 2013 Jul 3;154(1):228-39. doi: 10.1016/j.cell.2013.06.006.
  PUBMED    23827685
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "228-39"
COMMENT     Sequence Label: pECIA14
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    123..257
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(160..179)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     214..233
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     290..307
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(330..347)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(363..386)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgcctgcagg tcgactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
       61 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      121 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
      181 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      241 cttatcatgt ctggatcctc gatcgaggct gatcagcgag ctcaggcctt agaaggcaca
      301 gtcgaggctg atcagcgggt ttaaactcaa tggtgatggt gatgatgacc ggtgccgctg
      361 gccttatcat catcatcctt gtaatcgtta tcgccggagg tgcttcctct agaacccggg
      421 tgcgcggcgt cggtggtgcc ggcggggggc gccaggtcgc aggcggtgta gggctccagg
      481 caggcggcga aggccatgac gtgcgctatg aaggtctgct cctgcacgcc gtgaaccagg
      541 tgcgcctgcg ggccgcgcgc gaacaccgcc acgtcctcgc ctgcgtgggt ctcttcgtcc
      601 aggggcactg ctgactgctg ccgatactcg gggctcccgc tctcgctctc ggtaacatcc
      661 ggccgggcgc cgtccttgag cacatagcct ggaccgtttc cgtataggag gaccgtgtag
      721 gccttcctgt cccgggcctt gccaggggcc agcccgaaga tggagctccc tcgcaggggg
      781 tagcctccga aggagaagac gtgggagtgg tcggcagtga cgaggctcag cgtgtcctcc
      841 tcgctggtga gctggcccgc cctctcaatg gcgtcgtcga acatgatcgt ctcagtcagt
      901 gcccggtaag ccctgctttc atgatgacca tggtcgatgc gac
//
