LOCUS       sequence-69482-e        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring BBa_B1006 U10 termination 
            efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB1907
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(106..200)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(231..1025)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(342..361)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     952..971
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc
       61 acctgaagtc agccccatac gatataagtt gttactagtg cttggattct caccaataaa
      121 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
      181 tggatctatc aacaggagtc caagcgagct ctcgaacccc agagtcccgc tcagaagaac
      241 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
      301 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
      361 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
      421 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
      481 tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
      541 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
      601 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
      661 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
      721 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
      781 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
      841 tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
      901 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
      961 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     1021 atcatgcgaa a
//
